Lesson Plan: B1.3.05

• Buffer: Solution at a set pH and remains at that pH. (1)
• Rate of reaction: How fast a reaction takes place. (1)
• pH: How acidic or alkaline a substance is. (1)
• Enzyme: Proteins that act as biological catalysts, each specific for a certain metabolic reaction. (1)

Amylase soln 1% starch soln 1%, iodine soln water bath 40c 2 racks thermometers stopclock pipettes 10ml cylinders spotting tiles test tubes pH Range (4, 7, 9) 1. Measure 10 cm3 of starch solution using the 10 cm3 plastic syringe and place into the boiling tube. 2. Measure 1 cm3 of buffer solution using the 1 cm3 plastic syringe then add this to the starch solution. 3. Measure 1 cm3 of amylase solution using the 1 cm3 plastic syringe then add this to the test tube. 4. Place both tubes into the beaker of water to warm up. 5. Put one drop of iodine solution into each well of the spotting tile. 6. Add the amylase solution to the starch solution and mix. 7. Take out a drop of the starch amylase mixture and add to a well in the spotting tile. 8. Repeat this every 30 seconds until there is no change in colour or 5 minutes has passed. 9. Repeat steps 1 to 8 for different pH values.