Milk Bactoscan Method

1. Label an agar plate with your name, date and 1 / 100
2. In a linear air flow sterile pod
3. Use serial dilution to produce 1 / 100 milk dilution:
   • Measure 1 ml of neat milk using a pipette filler
   • Add to 9 ml of sterile, distilled water, to produce 1 / 10 milk dilution
   • Measure 1 ml of 1 / 10 milk dilution using a pipette filler
   • Add to 9 ml of sterile, distilled water, to produce 1 / 100 milk dilution
4. Measure 0.1 ml of 1 / 100 milk dilution using a pipette filler
5. Put the 1 / 100 milk dilution on to an agar plate
6. Spread out the milk using a glass spreader
7. Secure the agar plate closed with an elastic band
8. Incubate for 48 hours
9. Count the number of cultures and calculate the amount of bacteria in 1 ml of milk

Total Bateria Count (TBC)

• Each cell in the sample grows into one colony (cultures) on the plate.
• TBC is measured as bacterial per mililitre, the following calculation is needed.

FORMULA:
Number of colonies × dilution × 10 = TBC / ml

EXAMPLE:
25 colonies × 100 × 10 = 2,5000 cells in 1ml of neat milk

Pay Scale Based on Cell Count
Category 1	Less that 200,000 cells	no deduction
Category 2	200,001 to 250,000 cells	a deduction of 0.2p a litre
Category 3	250,001 to 400,000 cells	a deduction of 0.5p a litre
Category 4	Over 400,000 cells	the dairy will not buy the milk